1. 基本信(xin)息
品系名称:C57BL/6-Gt(ROSA)26Sortm1(CAG-tdTomato)/Vst
常用名:B6-tdToamto; tdToamto小鼠
背景:C57BL/6NCrl
毛色:黑色
品系建立:
在Rosa26位点定点敲(qiao)入CAG-tdToamto序列,获得(de)全(quan)身(shen)表达(da)红色荧光的首建(jian)鼠(shu)。首建(jian)鼠(shu)与野生(sheng)型(xing)C57BL/6NCrl交配(pei)获得(de)杂合鼠(shu),杂合鼠(shu)互配(pei)获得(de)纯合鼠(shu)。
2. 表型(xing)
除红(hong)细胞和毛发外,该小鼠品(pin)系的细胞可以激发出(chu)红(hong)色(se)荧光(tdToamto).
3. 应(ying)用领域
小(xiao)鼠(shu)细(xi)胞或组织追踪;另外分离自tdToamto红色(se)荧光小(xiao)鼠(shu)的细(xi)胞可能(neng)比转染(ran)EGFP质(zhi)粒的野生型小(xiao)鼠(shu)原代细(xi)胞更容易培养。
4. 参考文献
(1)Madisen L; Zwingman TA; Sunkin SM; Oh SW; Zariwala HA; Gu H; Ng LL; Palmiter RD; Hawrylycz MJ; Jones AR; Lein ES; Zeng H. 2010. A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci 13(1):133-40.
(2)Strathdee D, Ibbotson H, Grant SG. Expression of transgenes targeted to the Gt(ROSA)26Sor locus is orientation dependent. PLoS One. 2006 Dec 20;1:e4.